Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5044
|
Name |
pilM
|
Replicon | chromosome |
Genomic location | 5679649 - 5680713 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253731.1 |
GI | 15600237 |
Affymetrix | PA5044_pilM_at |
Entrez | 881162 |
GenBank | AAG08429.1 |
INSDC | AAG08429.1 |
NCBI Locus Tag | PA5044 |
protein_id(GenBank) | gb|AAG08429.1|AE004917_8|gnl|PseudoCAP|PA5044 |
TIGR | NTL03PA05045 |
UniParc | UPI00000D4249 |
UniProtKB Acc | G3XD28 |
UniProtKB ID | G3XD28_PSEAE |
UniRef100 | UniRef100_W0Z0P4 |
UniRef50 | UniRef50_A0A078M0F9 |
UniRef90 | UniRef90_A6VDG5 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
type 4 fimbrial biogenesis protein PilM
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -11.81 |
Kyte-Doolittle Hydrophobicity Value | 0.085 |
Molecular Weight (kDa) | 38.0 |
Isoelectric Point (pI) | 4.54 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5EQ6 | PEPTIDE BINDING PROTEIN | 11/12/15 | Pseudomonas aeruginosa PilM bound to AMP-PNP | Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 3.5 | X-RAY DIFFRACTION | 100.0 |
5EOX | PEPTIDE BINDING PROTEIN | 11/10/15 | Pseudomonas aeruginosa PilM bound to ADP | Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 2.4 | X-RAY DIFFRACTION | 100.0 |
5EOY | PEPTIDE BINDING PROTEIN | 11/10/15 | Pseudomonas aeruginosa SeMet-PilM bound to ADP | Pseudomonas aeruginosa | 2.5 | X-RAY DIFFRACTION | 100.0 |
5EOU | PEPTIDE BINDING PROTEIN | 11/10/15 | Pseudomonas aeruginosa PilM:PilN1-12 bound to ATP | Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) | 2.4 | X-RAY DIFFRACTION | 100.0 |
Source 1 | |
Database | VFDB : VF0082 |
Category | Type IV pili biosynthesis |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 216 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004647 (491 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5044
Search term: pilM
Search term: type 4 fimbrial biogenesis protein PilM
|
Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.
Martin PR, Watson AA, McCaul TF, Mattick JS
Mol Microbiol 1995 May;16(3):497-508
PubMed ID: 7565110
|
Characterization of genes required for pilus expression in Pseudomonas syringae pathovar phaseolicola.
Roine E, Nunn DN, Paulin L, Romantschuk M
J Bacteriol 1996 Jan;178(2):410-7
PubMed ID: 8550460
|
PilM/N/O/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin.
Ayers M, Sampaleanu LM, Tammam S, Koo J, Harvey H, Howell PL, Burrows LL
J Mol Biol 2009 Nov 20;394(1):128-42
PubMed ID: 19857645
|