Pseudomonas aeruginosa PAO1, PA5257

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5257
Name
Synonym: hemY
Replicon chromosome
Genomic location 5918350 - 5919588 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253944.1
GI 15600450
Affymetrix PA5257_at
Entrez 879722
GenBank AAG08642.1
INSDC AAG08642.1
NCBI Locus Tag PA5257
protein_id(GenBank) gb|AAG08642.1|AE004938_4|gnl|PseudoCAP|PA5257
TIGR NTL03PA05258
UniParc UPI00000C5F78
UniProtKB Acc Q9HTT8
UniProtKB ID Q9HTT8_PSEAE
UniRef100 UniRef100_Q9HTT8
UniRef50 UniRef50_A4VGY2
UniRef90 UniRef90_Q9HTT8

Product

Feature Type CDS
Coding Frame 1
Product Name
hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (46.64 kDa, PMID:21751344)
Charge (pH 7) 5.85
Kyte-Doolittle Hydrophobicity Value -0.197
Molecular Weight (kDa) 46.6
Isoelectric Point (pI) 9.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 111 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004838 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5257

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The sequence of hemC, hemD and two additional E. coli genes.
Alefounder PR, Abell C, Battersby AR
Nucleic Acids Res 1988 Oct 25;16(20):9871
PubMed ID: 3054815
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344