Pseudomonas aeruginosa PAO1, PA0102 (psCA1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0102
Name
psCA1
Replicon chromosome
Genomic location 123871 - 124599 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248792.1
GI 15595300
Affymetrix PA0102_at
DNASU PaCD00006044
Entrez 880767
GenBank AAG03492.1
INSDC AAG03492.1
NCBI Locus Tag PA0102
protein_id(GenBank) gb|AAG03492.1|AE004449_1|gnl|PseudoCAP|PA0102
TIGR NTL03PA00103
UniParc UPI00000C4F37
UniProtKB Acc Q9I730
UniProtKB ID Q9I730_PSEAE
UniRef100 UniRef100_Q9I730
UniRef50 UniRef50_Q9I730
UniRef90 UniRef90_Q9I730

Product

Feature Type CDS
Coding Frame 1
Product Name
beta-carbonic anhydrase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by iTRAQ (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.75
Kyte-Doolittle Hydrophobicity Value -0.219
Molecular Weight (kDa) 26.6
Isoelectric Point (pI) 6.67

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 421 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000100 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0102
Search term: psCA1

Human Homologs

References

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Three functional β-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air.
Lotlikar SR, Hnatusko S, Dickenson NE, Choudhari SP, Picking WL, Patrauchan MA
Microbiology (Reading, Engl.) 2013 Aug;159(Pt 8):1748-59
PubMed ID: 23728627
Pseudomonas aeruginosa β-carbonic anhydrase, psCA1, is required for calcium deposition and contributes to virulence.
Lotlikar SR, Kayastha BB, Vullo D, Khanam SS, Braga RE, Murray AB, McKenna R, Supuran CT, Patrauchan MA
Cell Calcium 2019 Dec;84:102080
PubMed ID: 31589941