Pseudomonas aeruginosa PAO1, PA0681 (hxcT)

Annotation Updates

Date	Description	Acknowledgements
May 12, 2006	New structure feature: Manually-identified prepilin peptidase substrate (type IV) export signal (MDVVQFSSSPKGHRGQRGFTLIE)	Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Jun 10, 2004	Annotation of gene previously described as probable type II secretion system protein (Class 3). New protein name: HxcT pseudopilin. New protein name confidence: Class 1. New evidence reference (PubMed ID): 11985723. New homology added: 68% identical and 80% similar to GspG[Burkholderia pseudomallei];60% identical and 76% similar to XcpT[Pseudomonas alcaligenes];57% identical and 70% similar to XcpT[Pseudomonas aeruginosa]. New genomic context added: Ribosome binding site: GAGGAG from -13 to -8 (Kolaskar and Reddy rule for ATG: 27). Good translation start site. In agreement with the fact that in the Xcp system XcpT is the most abundantly produced pseudopilin. Part of the hxcTXYZQRS operon. New structure features added: The leader peptide extends between residues 1 to 18, and includes the invariant glycine after which cleavage by the prepilin peptidase takes place. The new N-terminal residue is phenylalanine, and precedes 21 highly hydrophobic residues. New comments: The fifth residue of the mature sequence is a glutamate, which seems important in the methylation of this class of proteins.hxc gene cluster is under phosphate regulation. Belongs to the GspG family (general secretory pathway).	Raymond C. Lo and Fiona S.L. Brinkman, Simon Fraser University, Canada (based on literature review);
May 8, 2015	New gene name: hxcT. New product name: HxcT.	Rome Voulhoux, CNRS, Marseille, France