Pseudomonas aeruginosa PAO1, PA0008 (glyS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0008
Name
glyS
Replicon chromosome
Genomic location 10434 - 12488 (- strand)

Cross-References

RefSeq NP_064728.1
GI 15595206
Affymetrix PA0008_glyS_at
Entrez 878214
GenBank AAG03398.1
INSDC AAG03398.1
NCBI Locus Tag PA0008
protein_id(GenBank) gb|AAG03398.1|AE004440_8|gnl|PseudoCAP|PA0008
TIGR NTL03PA00009
UniParc UPI00001364A2
UniProtKB Acc Q9I7B8
UniProtKB ID SYGB_PSEAE
UniRef100 UniRef100_Q9I7B8
UniRef50 UniRef50_Q4KKR8
UniRef90 UniRef90_Q9I7B8

Product

Feature Type CDS
Coding Frame 1
Product Name
glycyl-tRNA synthetase beta chain
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -15.14
Kyte-Doolittle Hydrophobicity Value -0.032
Molecular Weight (kDa) 74.0
Isoelectric Point (pI) 4.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 403 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000008 (574 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0008
Search term: glyS

References

Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame.
Keng T, Webster TA, Sauer RT, Schimmel P.
J Biol Chem. 1982 Nov 10;257(21):12503-8.
PubMed ID: 6290471
Primary structures of both subunits of Escherichia coli glycyl-tRNA synthetase.
Webster TA, Gibson BW, Keng T, Biemann K, Schimmel P.
J Biol Chem. 1983 Sep 10;258(17):10637-41.
PubMed ID: 6309809
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM.
Proteomics. 2009 Apr;9(7):1901-15.
PubMed ID: 19333994