Pseudomonas aeruginosa PAO1, PA0076 (tagF1)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 90844 - 91524 (- strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_248766.1
GI 15595274
Affymetrix PA0076_at
DNASU PaCD00006034
Entrez 879335
GenBank AAG03466.1
INSDC AAG03466.1
NCBI Locus Tag PA0076
protein_id(GenBank) gb|AAG03466.1|AE004446_14|gnl|PseudoCAP|PA0076
UniParc UPI00000C4F1D
UniProtKB Acc Q9I756
UniRef100 UniRef100_Q9I756
UniRef50 UniRef50_Q4K3P5
UniRef90 UniRef90_Q9I756


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.61
Kyte-Doolittle Hydrophobicity Value 0.146
Molecular Weight (kDa) 23.9
Isoelectric Point (pI) 4.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2QNU STRUCTURAL GENOMICS, UNKNOWN FUNCTION 07/19/07 Crystal structure of PA0076 from Pseudomonas aeruginosa PAO1 at 2.05 A resolution PSEUDOMONAS AERUGINOSA PAO1 2.05 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Reference 16763151

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 49 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000075 (394 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0076
Search term: tagF1
Search term: TagF1

Transposon Insertions in PAO1

Type mini-Tn5 lux tranposon insertion site
Mutant ID UBC: PAO1_lux_12_D1
Genomic Position 90844
Position in ORF 1(681)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 52071
Genomic Position 91306
Position in ORF 463(681)
Reference 14617778

Transposon Insertions in Orthologs


A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433