Pseudomonas aeruginosa PAO1, PA0158 (triC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0158
Name
triC
Synonym: triC
Replicon chromosome
Genomic location 179522 - 182569 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1

Cross-References

RefSeq NP_248848.1
GI 15595356
Affymetrix PA0158_at
Entrez 882140
GenBank AAG03548.1
INSDC AAG03548.1
NCBI Locus Tag PA0158
protein_id(GenBank) gb|AAG03548.1|AE004453_11|gnl|PseudoCAP|PA0158
TIGR NTL03PA00159
UniParc UPI00000C4F6D
UniProtKB Acc Q9I6X4
UniProtKB ID Q9I6X4_PSEAE
UniRef100 UniRef100_U8MJ39
UniRef50 UniRef50_F6A9K9
UniRef90 UniRef90_W0YJL0

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) triclosan efflux transporter, TriC
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.33
Kyte-Doolittle Hydrophobicity Value 0.184
Molecular Weight (kDa) 111.9
Isoelectric Point (pI) 5.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
TriC TriC is a resistance nodulation cell division (RND) transporter that is a part of TriABC-OpmH, a triclosan-specific efflux protein. ARO:3003681 protein homolog model Perfect 0.0 1.0 1.1.8

Virulence Evidence

Source 1
Database Victors : 4263
Category Not available / Not known
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 12023080

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000156 (1046 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0158
Search term: triC

Transposon Insertions in PAO1

Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 8674
Genomic Position 181573
Position in ORF 2052(3048)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 33573
Genomic Position 180161
Position in ORF 640(3048)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 31958
Genomic Position 181724
Position in ORF 2203(3048)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_82_E6
Genomic Position 181511
Position in ORF 1990(3048)
Reference 15805499

Transposon Insertions in Orthologs

References

Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins.
Mima T, Joshi S, Gomez-Escalada M, Schweizer HP
J. Bacteriol. 2007 Nov;189(21):7600-9
PubMed ID: 17720796