Pseudomonas aeruginosa PAO1, PA0183 (atsA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0183
Name
atsA
Replicon chromosome
Genomic location 207923 - 209533 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248873.1
GI 15595381
Affymetrix PA0183_atsA_at
Entrez 879288
GenBank AAG03573.1
INSDC AAG03573.1
NCBI Locus Tag PA0183
protein_id(GenBank) gb|AAG03573.1|AE004456_4|gnl|PseudoCAP|PA0183
TIGR NTL03PA00184
UniParc UPI0000165EBE
UniProtKB Acc P51691
UniProtKB ID ARS_PSEAE
UniRef100 UniRef100_P51691
UniRef50 UniRef50_P51691
UniRef90 UniRef90_P51691

Product

Feature Type CDS
Coding Frame 1
Product Name
arylsulfatase
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -12.39
Kyte-Doolittle Hydrophobicity Value -0.378
Molecular Weight (kDa) 59.9
Isoelectric Point (pI) 5.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4CXK HYDROLASE 04/07/14 G9 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 1.86 X-RAY DIFFRACTION 98.1
4CYS HYDROLASE 04/14/14 G6 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid PSEUDOMONAS AERUGINOSA 1.88 X-RAY DIFFRACTION 98.7
4CYR HYDROLASE 04/14/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 1.72 X-RAY DIFFRACTION 98.7
4CXS HYDROLASE 04/08/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 98.7
5AJ9 HYDROLASE 02/20/15 G7 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 98.5
4CXU HYDROLASE 04/08/14 G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with 3-Br-Phenolphenylphosphonate PSEUDOMONAS AERUGINOSA 2.03 X-RAY DIFFRACTION 98.7
1HDH HYDROLASE 11/16/00 Arylsulfatase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.3 X-RAY DIFFRACTION 99.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 242 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000181 (382 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0183
Search term: atsA
Search term: arylsulfatase

Human Homologs

Ensembl 86, assembly GRCh38.p7
arylsulfatase B [Source:HGNC Symbol;Acc:HGNC:714]
E-value: 1.6e-27
Percent Identity: 34.8

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 19684
Genomic Position 208954
Position in ORF 1032(1611)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 41442
Genomic Position 208707
Position in ORF 785(1611)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (exoU background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02310
Mutant ID PA14NR: 6402
Genomic Position 207712
Position in ORF 929(1611)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02310
Mutant ID PA14NR: 43813
Genomic Position 207329
Position in ORF 546(1611)
Reference 16477005

References

Purification and characterization of the arylsulfatase synthesized by Pseudomonas aeruginosa PAO during growth in sulfate-free medium and cloning of the arylsulfatase gene (atsA).
Beil S, Kehrli H, James P, Staudenmann W, Cook AM, Leisinger T, Kertesz MA.
Eur J Biochem. 1995 Apr 15;229(2):385-94.
PubMed ID: 7744061