Pseudomonas aeruginosa PAO1, PA0215

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0215
Name
Synonym: madL
Replicon chromosome
Genomic location 243431 - 243835 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_248906.1
GI 15595412
Affymetrix PA0215_at
Entrez 883110
GenBank AAG03604.1
INSDC AAG03604.1
NCBI Locus Tag PA0215
protein_id(GenBank) gb|AAG03604.1|AE004459_8|gnl|PseudoCAP|PA0215
TIGR NTL03PA00216
UniParc UPI00000C4F9E
UniProtKB Acc Q9I6S3
UniProtKB ID Q9I6S3_PSEAE
UniRef100 UniRef100_Q9I6S3
UniRef50 UniRef50_Q87V61
UniRef90 UniRef90_A6UY10

Product

Feature Type CDS
Coding Frame 1
Product Name
malonate transporter MadL
Product Name Confidence: Class 2
CommentBIOLOG data shows growth on malonate. Annotation assigns PA0215 and PA0216 as having 71% and 81% homology with MadL and MadM from Malonomonas rubra.
Evidence for Translation
Charge (pH 7) 4.95
Kyte-Doolittle Hydrophobicity Value 1.019
Molecular Weight (kDa) 13.6
Isoelectric Point (pI) 10.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 30 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000214 (490 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0215

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 21867
Genomic Position 243503
Position in ORF 73(405)
Reference 14617778

Transposon Insertions in Orthologs

References

Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.
Berg M, Hilbi H, Dimroth P
Eur. J. Biochem. 1997 Apr;245(1):103-15
PubMed ID: 9128730
Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes.
Schaffitzel C, Berg M, Dimroth P, Pos KM
J. Bacteriol. 1998 May;180(10):2689-93
PubMed ID: 9573154