Pseudomonas aeruginosa PAO1, PA2579 (kynA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2579
Name
kynA
Replicon chromosome
Genomic location 2914966 - 2915832 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentGenetic evidence.

Cross-References

RefSeq NP_251269.1
GI 15597775
Affymetrix PA2579_at
DNASU PaCD00007113
Entrez 879140
GenBank AAG05967.1
INSDC AAG05967.1
NCBI Locus Tag PA2579
protein_id(GenBank) gb|AAG05967.1|AE004686_5|gnl|PseudoCAP|PA2579
TIGR NTL03PA02579
UniParc UPI00000C574D
UniProtKB Acc Q9I0Q7
UniProtKB ID T23O_PSEAE
UniRef100 UniRef100_Q9I0Q7
UniRef50 UniRef50_Q1LK00
UniRef90 UniRef90_Q9I0Q7

Product

Feature Type CDS
Coding Frame 1
Product Name
L-Tryptophan:oxygen 2,3-oxidoreductase (decyclizing) KynA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.21
Kyte-Doolittle Hydrophobicity Value -0.310
Molecular Weight (kDa) 32.9
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 136 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002967 (352 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2579
Search term: kynA

Human Homologs

Ensembl 110, assembly GRCh38.p14
tryptophan 2,3-dioxygenase [Source:HGNC Symbol;Acc:HGNC:11708]
E-value: 3.3e-16
Percent Identity: 43.2
Ensembl 110, assembly GRCh38.p14
tryptophan 2,3-dioxygenase [Source:HGNC Symbol;Acc:HGNC:11708]
E-value: 3.3e-16
Percent Identity: 43.2

References

Two distinct pathways supply anthranilate as a precursor of the Pseudomonas quinolone signal.
Farrow JM 3rd, Pesci EC
J Bacteriol 2007 May;189(9):3425-33
PubMed ID: 17337571
Aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase.
Kurnasov O, Jablonski L, Polanuyer B, Dorrestein P, Begley T, Osterman A
FEMS Microbiol Lett 2003 Oct 24;227(2):219-27
PubMed ID: 14592712