Pseudomonas aeruginosa PAO1, PA2586 (gacA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2586
Name
gacA
Replicon chromosome
Genomic location 2925788 - 2926432 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251276.1
GI 15597782
Affymetrix PA2586_gacA_at
Entrez 880582
GenBank AAG05974.1
INSDC AAG05974.1
NCBI Locus Tag PA2586
protein_id(GenBank) gb|AAG05974.1|AE004687_3|gnl|PseudoCAP|PA2586
TIGR NTL03PA02586
UniParc UPI000012AFCE
UniProtKB Acc Q51373
UniProtKB ID GACA_PSEAE
UniRef100 UniRef100_Q51373
UniRef50 UniRef50_Q51373
UniRef90 UniRef90_Q51373

Product

Feature Type CDS
Coding Frame 1
Product Name
response regulator GacA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.19
Kyte-Doolittle Hydrophobicity Value 0.040
Molecular Weight (kDa) 23.6
Isoelectric Point (pI) 6.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category GacS/GacA two-component system
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Bombyx mori
Notes gacA mutant strains exhibit reduced killing of silkworm.
Reference 15727838
Source 2
Database VFDB
Category GacS/GacA two-component system
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 606 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002960 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2586
Search term: gacA

Human Homologs

References

The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.
Reimmann C, Beyeler M, Latifi A, Winteler H, Foglino M, Lazdunski A, Haas D
Mol Microbiol 1997 Apr;24(2):309-19
PubMed ID: 9159518