Pseudomonas aeruginosa PAO1, PA2643 (nuoH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2643
Name
nuoH
Replicon chromosome
Genomic location 2990435 - 2991430 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251333.1
GI 15597839
Affymetrix PA2643_nuoH_at
DNASU PaCD00007139
Entrez 882350
GenBank AAG06031.1
INSDC AAG06031.1
NCBI Locus Tag PA2643
protein_id(GenBank) gb|AAG06031.1|AE004693_2|gnl|PseudoCAP|PA2643
TIGR NTL03PA02643
UniParc UPI00000C5784
UniProtKB Acc Q9I0J5
UniProtKB ID NUOH_PSEAE
UniRef100 UniRef100_B7VAQ9
UniRef50 UniRef50_P57258
UniRef90 UniRef90_B7VAQ9

Product

Feature Type CDS
Coding Frame 1
Product Name
NADH dehydrogenase I chain H
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.14
Kyte-Doolittle Hydrophobicity Value 0.867
Molecular Weight (kDa) 36.7
Isoelectric Point (pI) 6.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 489 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002911 (518 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2643
Search term: nuoH

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1 [Source:HGNC Symbol;Acc:HGNC:7455]
E-value: 7.4e-44
Percent Identity: 36.6

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I.
Weidner U, Geier S, Ptock A, Friedrich T, Leif H, Weiss H
J Mol Biol 1993 Sep 5;233(1):109-22
PubMed ID: 7690854