Pseudomonas aeruginosa PAO1, PA2654 (tlpQ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2654
Name
tlpQ
Replicon chromosome
Genomic location 3003457 - 3005601 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 5 transposon mutants in orthologs

Cross-References

RefSeq NP_251344.1
GI 15597850
Affymetrix PA2654_at
Entrez 882363
GenBank AAG06042.1
INSDC AAG06042.1
NCBI Locus Tag PA2654
protein_id(GenBank) gb|AAG06042.1|AE004694_5|gnl|PseudoCAP|PA2654
TIGR NTL03PA02654
UniParc UPI00000C578E
UniProtKB Acc Q9I0I4
UniProtKB ID Q9I0I4_PSEAE
UniRef100 UniRef100_S0IHM2
UniRef50 UniRef50_Q4K9H3
UniRef90 UniRef90_A6V4C5

Product

Feature Type CDS
Coding Frame 1
Product Name
TlpQ
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -22.11
Kyte-Doolittle Hydrophobicity Value -0.134
Molecular Weight (kDa) 77.1
Isoelectric Point (pI) 4.68

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6FU4 SIGNALING PROTEIN 02/26/18 Ligand binding domain (LBD) of the p. aeruginosa histamine receptor TlpQ Pseudomonas aeruginosa PAO1 2.45 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 403 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002657 (1774 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2654
Search term: tlpQ
Search term: TlpQ

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Genetic identification of chemotactic transducers for amino acids in Pseudomonas aeruginosa.
Taguchi K, Fukutomi H, Kuroda A, Kato J, Ohtake H
Microbiology (Reading) 1997 Oct;143 ( Pt 10):3223-3229
PubMed ID: 9353923
High-Affinity Chemotaxis to Histamine Mediated by the TlpQ Chemoreceptor of the Human Pathogen Pseudomonas aeruginosa.
Corral-Lugo A, Matilla MA, Martín-Mora D, Silva Jiménez H, Mesa Torres N, Kato J, Hida A, Oku S, Conejero-Muriel M, Gavira JA, Krell T
mBio 2018 Nov 13;9(6)
PubMed ID: 30425146