Pseudomonas aeruginosa PAO1, PA2741 (rplT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2741
Name
rplT
Replicon chromosome
Genomic location 3103643 - 3103999 (- strand)

Cross-References

RefSeq NP_251431.1
GI 15597937
Affymetrix PA2741_rplT_at
DNASU PaCD00007186
Entrez 882982
GenBank AAG06129.1
INSDC AAG06129.1
NCBI Locus Tag PA2741
protein_id(GenBank) gb|AAG06129.1|AE004702_6|gnl|PseudoCAP|PA2741
TIGR NTL03PA02741
UniParc UPI0000133D1E
UniProtKB Acc Q9I0A2
UniProtKB ID RL20_PSEAE
UniRef100 UniRef100_A6V488
UniRef50 UniRef50_A0QHC1
UniRef90 UniRef90_C1DF45

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L20
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (13.36 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Charge (pH 7) 26.22
Kyte-Doolittle Hydrophobicity Value -0.355
Molecular Weight (kDa) 13.4
Isoelectric Point (pI) 12.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002830 (529 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2741
Search term: rplT

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L20 [Source:HGNC Symbol;Acc:HGNC:14478]
E-value: 3.8e-11
Percent Identity: 38.6

References

Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon.
Springer M, Mayaux JF, Fayat G, Plumbridge JA, Graffe M, Blanquet S, Grunberg-Manago M
J Mol Biol 1985 Feb 20;181(4):467-78
PubMed ID: 3158742
Suppression of a sensor kinase-dependent phenotype in Pseudomonas syringae by ribosomal proteins L35 and L20.
Kitten T, Willis DK
J Bacteriol 1996 Mar;178(6):1548-55
PubMed ID: 8626280
Physiological effects of translation initiation factor IF3 and ribosomal protein L20 limitation in Escherichia coli.
Olsson CL, Graffe M, Springer M, Hershey JW
Mol Gen Genet 1996 Apr 10;250(6):705-14
PubMed ID: 8628231
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344