Pseudomonas aeruginosa PAO1, PA2755 (eco)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2755
Name
eco
Replicon chromosome
Genomic location 3116654 - 3117124 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_251445.1
GI 15597951
Affymetrix PA2755_eco_at
DNASU PaCD00007195
Entrez 882725
GenBank AAG06143.1
INSDC AAG06143.1
NCBI Locus Tag PA2755
protein_id(GenBank) gb|AAG06143.1|AE004703_9|gnl|PseudoCAP|PA2755
TIGR NTL03PA02755
UniParc UPI0000129B88
UniProtKB Acc Q9I088
UniProtKB ID ECOT_PSEAE
UniRef100 UniRef100_Q9I088
UniRef50 UniRef50_Q9I088
UniRef90 UniRef90_Q9I088

Product

Feature Type CDS
Coding Frame 1
Product Name
ecotin precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.62
Kyte-Doolittle Hydrophobicity Value -0.206
Molecular Weight (kDa) 17.2
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Extracellular Class 1
Western blot evidence
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 38 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002813 (409 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2755
Search term: eco
Search term: ecotin precursor

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The sequence and reactive site of ecotin. A general inhibitor of pancreatic serine proteases from Escherichia coli.
McGrath ME, Hines WM, Sakanari JA, Fletterick RJ, Craik CS
J Biol Chem 1991 Apr 5;266(10):6620-5
PubMed ID: 2007606
Ecotin: lessons on survival in a protease-filled world.
McGrath ME, Gillmor SA, Fletterick RJ
Protein Sci 1995 Feb;4(2):141-8
PubMed ID: 7757004
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
A Biofilm Matrix-Associated Protease Inhibitor Protects Pseudomonas aeruginosa from Proteolytic Attack.
Tseng BS, Reichhardt C, Merrihew GE, Araujo-Hernandez SA, Harrison JJ, MacCoss MJ, Parsek MR
mBio 2018 Apr 10;9(2)
PubMed ID: 29636440