Pseudomonas aeruginosa PAO1, PA2840

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2840
Name
Synonym: deaD
Replicon chromosome
Genomic location 3193886 - 3195589 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251530.1
GI 15598036
Affymetrix PA2840_at
Entrez 882823
GenBank AAG06228.1
INSDC AAG06228.1
NCBI Locus Tag PA2840
protein_id(GenBank) gb|AAG06228.1|AE004710_10|gnl|PseudoCAP|PA2840
TIGR NTL03PA02840
UniParc UPI00000C5838
UniProtKB Acc Q9I003
UniProtKB ID Q9I003_PSEAE
UniRef100 UniRef100_Q9I003
UniRef50 UniRef50_Q4K8U0
UniRef90 UniRef90_Q9I003

Product

Feature Type CDS
Coding Frame 1
Product Name
probable ATP-dependent RNA helicase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (62.11 kDa, PMID:21751344)
Charge (pH 7) 8.99
Kyte-Doolittle Hydrophobicity Value -0.209
Molecular Weight (kDa) 62.1
Isoelectric Point (pI) 9.02

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 637 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002728 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2840

Human Homologs

Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 23 [Source:HGNC Symbol;Acc:HGNC:17347]
E-value: 8.5e-56
Percent Identity: 38.8
Ensembl 110, assembly GRCh38.p14
DEAD-box helicase 23 [Source:HGNC Symbol;Acc:HGNC:17347]
E-value: 8.5e-56
Percent Identity: 38.8

References

deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2.
Toone WM, Rudd KE, Friesen JD
J Bacteriol 1991 Jun;173(11):3291-302
PubMed ID: 2045359
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344