Pseudomonas aeruginosa PAO1, PA2854

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2854
Name
Synonym: erfK
Replicon chromosome
Genomic location 3207290 - 3208261 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 4LZH: L,D-transpeptidase from Klebsiella pneumoniae (root-mean-square deviation: 0.7 angstroms)

Cross-References

RefSeq NP_251544.1
GI 15598050
Affymetrix PA2854_at
DNASU PaCD00007243
Entrez 879852
GenBank AAG06242.1
INSDC AAG06242.1
NCBI Locus Tag PA2854
protein_id(GenBank) gb|AAG06242.1|AE004712_2|gnl|PseudoCAP|PA2854
TIGR NTL03PA02854
UniParc UPI00000C5845
UniProtKB Acc Q9HZZ0
UniProtKB ID Q9HZZ0_PSEAE
UniRef100 UniRef100_W0YRJ1
UniRef50 UniRef50_A0A0B7DBL0
UniRef90 UniRef90_A6V3N6

Product

Feature Type CDS
Coding Frame 1
Product Name
putative L,D-transpeptidase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -6.11
Kyte-Doolittle Hydrophobicity Value -0.140
Molecular Weight (kDa) 34.8
Isoelectric Point (pI) 5.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 204 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002715 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2854

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799