Pseudomonas aeruginosa PAO1, PA2950 (fabV)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA2950
Name	fabV Synonym: pfm
Replicon	chromosome
Genomic location	3309411 - 3310607 (- strand)
Comment	Confers resistance to triclosan.

Cross-References

RefSeq	NP_251640.1
GI	15598146
Affymetrix	PA2950_at
DNASU	PaCD00007286
Entrez	882931
GenBank	AAG06338.1
INSDC	AAG06338.1
NCBI Locus Tag	PA2950
protein_id(GenBank)	gb|AAG06338.1|AE004721_6|gnl|PseudoCAP|PA2950
TIGR	NTL03PA02950
UniParc	UPI00000C58A0
UniProtKB Acc	Q9HZP8
UniProtKB ID	Y2950_PSEAE
UniRef100	UniRef100_B7V2Z1
UniRef50	UniRef50_Q9PCE6
UniRef90	UniRef90_B7V2Z1

Product

Feature Type	CDS
Coding Frame	1
Product Name	FabV Product Name Confidence: Class 1
Synonyms	proton motive force protein, PFM enoyl-acyl-carrier protein reductase
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by iTRAQ (PMID:21863142).
Charge (pH 7)	-12.87
Kyte-Doolittle Hydrophobicity Value	-0.198
Molecular Weight (kDa)	43.5
Isoelectric Point (pI)	4.76

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database	PseudoCAP
Category
Evidence	ECO:0000314 direct assay evidence used in manual assertion
Host Organism	Caenorhabditis elegans
Notes
Infection model	C. elegans killing assay.
Reference	22092819

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 73 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002631 (408 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA2950 Search term: fabV Search term: FabV

Human Homologs

References

Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient. Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB Front Microbiol 2011;2:162 PubMed ID: 21863142

Identification and functional characterization of pfm, a novel gene involved in swimming motility of Pseudomonas aeruginosa. Bai F, Li Y, Xu H, Xia H, Yin T, Yao H, Zhang L, Zhang X, Bai Y, Jin S, Qiao M Gene 2007 Oct 15;401(1-2):19-27 PubMed ID: 17714889

Functional characterization of pfm in protein secretion and lung infection of Pseudomonas aeruginosa. Bai F, Xu H, Zhang Q, Qi X, Mou R, Bai G, Qiao M Can J Microbiol 2011 Oct;57(10):829-37 PubMed ID: 21950738

Mutation of pfm affects the adherence of Pseudomonas aeruginosa to host cells and the quorum sensing system. Mou R, Bai F, Duan Q, Wang X, Xu H, Bai Y, Zhang X, Jin S, Qiao M FEMS Microbiol Lett 2011 Nov;324(2):173-80 PubMed ID: 22092819

Triclosan resistance of Pseudomonas aeruginosa PAO1 is due to FabV, a triclosan-resistant enoyl-acyl carrier protein reductase. Zhu L, Lin J, Ma J, Cronan JE, Wang H Antimicrob Agents Chemother 2010 Feb;54(2):689-98 PubMed ID: 19933806

Functional Characterization of Triclosan-Resistant Enoyl-acyl-carrier Protein Reductase (FabV) in Pseudomonas aeruginosa. Huang YH, Lin JS, Ma JC, Wang HH Front Microbiol 2016;7:1903 PubMed ID: 27965638