Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2994
|
Name |
nqrF
|
Replicon | chromosome |
Genomic location | 3351656 - 3352879 (- strand) |
Transposon Mutants | 3 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_251684.1 |
GI | 15598190 |
Affymetrix | PA2994_nqrF_at |
Entrez | 878840 |
GenBank | AAG06382.1 |
INSDC | AAG06382.1 |
NCBI Locus Tag | PA2994 |
protein_id(GenBank) | gb|AAG06382.1|AE004724_11|gnl|PseudoCAP|PA2994 |
TIGR | NTL03PA02994 |
UniParc | UPI000013046C |
UniProtKB Acc | Q9HZL1 |
UniProtKB ID | NQRF_PSEAE |
UniRef100 | UniRef100_Q9HZL1 |
UniRef50 | UniRef50_A5F5Y4 |
UniRef90 | UniRef90_Q9HZL1 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Na+-translocating NADH:quinone oxidoreductase, subunit Nqr6
|
Synonyms | |
Evidence for Translation |
Detected using LC-MS/MS (45.47 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -13.57 |
Kyte-Doolittle Hydrophobicity Value | -0.186 |
Molecular Weight (kDa) | 45.4 |
Isoelectric Point (pI) | 4.77 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
7QU3 | FLAVOPROTEIN | 01/17/22 | X-ray structure of FAD domain of NqrF of Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.6 | X-RAY DIFFRACTION | 99.6 |
7QU5 | FLAVOPROTEIN | 01/17/22 | X-ray structure of FAD domain of NqrF of Pseudomonas aeruginosa | Pseudomonas aeruginosa | 1.25 | X-RAY DIFFRACTION | 99.6 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 426 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002587 (347 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2994
Search term: nqrF
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli.
Hayashi M, Hirai K, Unemoto T
FEBS Lett 1994 Dec 19;356(2-3):330-2
PubMed ID: 7805866
|
Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus.
Hayashi M, Hirai K, Unemoto T
FEBS Lett 1995 Apr 17;363(1-2):75-7
PubMed ID: 7729558
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|