Pseudomonas aeruginosa PAO1, PA2994 (nqrF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2994
Name
nqrF
Replicon chromosome
Genomic location 3351656 - 3352879 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251684.1
GI 15598190
Affymetrix PA2994_nqrF_at
Entrez 878840
GenBank AAG06382.1
INSDC AAG06382.1
NCBI Locus Tag PA2994
protein_id(GenBank) gb|AAG06382.1|AE004724_11|gnl|PseudoCAP|PA2994
TIGR NTL03PA02994
UniParc UPI000013046C
UniProtKB Acc Q9HZL1
UniProtKB ID NQRF_PSEAE
UniRef100 UniRef100_Q9HZL1
UniRef50 UniRef50_A5F5Y4
UniRef90 UniRef90_Q9HZL1

Product

Feature Type CDS
Coding Frame 1
Product Name
Na+-translocating NADH:quinone oxidoreductase, subunit Nqr6
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (45.47 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.57
Kyte-Doolittle Hydrophobicity Value -0.186
Molecular Weight (kDa) 45.4
Isoelectric Point (pI) 4.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Unknown (This protein may have multiple localization sites) Class 3
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7QU3 FLAVOPROTEIN 01/17/22 X-ray structure of FAD domain of NqrF of Pseudomonas aeruginosa Pseudomonas aeruginosa 1.6 X-RAY DIFFRACTION 99.6
7QU5 FLAVOPROTEIN 01/17/22 X-ray structure of FAD domain of NqrF of Pseudomonas aeruginosa Pseudomonas aeruginosa 1.25 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 426 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002587 (347 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2994
Search term: nqrF

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli.
Hayashi M, Hirai K, Unemoto T
FEBS Lett 1994 Dec 19;356(2-3):330-2
PubMed ID: 7805866
Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus.
Hayashi M, Hirai K, Unemoto T
FEBS Lett 1995 Apr 17;363(1-2):75-7
PubMed ID: 7729558
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344