Pseudomonas aeruginosa PAO1, PA2997 (nqrC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2997
Name
nqrC
Replicon chromosome
Genomic location 3354169 - 3354954 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251687.1
GI 15598193
Affymetrix PA2997_nqrC_at
DNASU PaCD00007309
Entrez 880204
GenBank AAG06385.1
INSDC AAG06385.1
NCBI Locus Tag PA2997
protein_id(GenBank) gb|AAG06385.1|AE004724_14|gnl|PseudoCAP|PA2997
TIGR NTL03PA02997
UniParc UPI0000130448
UniProtKB Acc Q9HZK8
UniProtKB ID NQRC_PSEAE
UniRef100 UniRef100_Q9HZK8
UniRef50 UniRef50_A5F5Y7
UniRef90 UniRef90_Q9HZK8

Product

Feature Type CDS
Coding Frame 1
Product Name
Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq3
Product Name Confidence: Class 2
Synonyms Na+-translocating NADH:ubiquinone oxidoreductase gamma subunit
Evidence for Translation
Detected using LC-MS/MS (27.78 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.31
Kyte-Doolittle Hydrophobicity Value -0.014
Molecular Weight (kDa) 27.8
Isoelectric Point (pI) 5.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 135 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002584 (339 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2997
Search term: nqrC

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and sequencing of four structural genes for the Na(+)-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus.
Beattie P, Tan K, Bourne RM, Leach D, Rich PR, Ward FB
FEBS Lett 1994 Dec 19;356(2-3):333-8
PubMed ID: 7805867
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344