Pseudomonas aeruginosa PAO1, PA3302 (phaJ1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3302
Name
phaJ1
Replicon chromosome
Genomic location 3700316 - 3700786 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_251992.1
GI 15598498
Affymetrix PA3302_at
Entrez 882467
GenBank AAG06690.1
INSDC AAG06690.1
NCBI Locus Tag PA3302
protein_id(GenBank) gb|AAG06690.1|AE004752_6|gnl|PseudoCAP|PA3302
TIGR NTL03PA03302
UniParc UPI00000C4ED0
UniProtKB Acc G3XD52
UniProtKB ID G3XD52_PSEAE
UniRef100 UniRef100_W0YTY3
UniRef50 UniRef50_A9Z1C1
UniRef90 UniRef90_A9Z1C1

Product

Feature Type CDS
Coding Frame 1
Product Name
PhaJ1
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.53
Kyte-Doolittle Hydrophobicity Value -0.057
Molecular Weight (kDa) 16.9
Isoelectric Point (pI) 5.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5CPG LYASE 07/21/15 R-Hydratase PhaJ1 from Pseudomonas aeruginosa in the unliganded form Pseudomonas aeruginosa 1.694 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 278 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002315 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3302
Search term: phaJ1
Search term: PhaJ1

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Contribution of the distal pocket residue to the acyl-chain-length specificity of (R)-specific enoyl-coenzyme A hydratases from Pseudomonas spp.
Tsuge T, Sato S, Hiroe A, Ishizuka K, Kanazawa H, Shiro Y, Hisano T
Appl Environ Microbiol 2015 Dec;81(23):8076-83
PubMed ID: 26386053