Pseudomonas aeruginosa PAO1, PA3479 (rhlA)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
| Locus Tag |
PA3479
|
| Name |
rhlA
|
| Replicon | chromosome |
| Genomic location | 3892121 - 3893008 (- strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
| Comment | RhlA condenses two beta-hyroxydecanoly-ACPs to form the beta-hydroxydecanoly-beta-hydroxyldecanoate moiety of rhamnolipids |
Cross-References
| RefSeq | NP_252169.1 |
| GI | 15598675 |
| Affymetrix | PA3479_rhlA_at |
| DNASU | PaCD00007528 |
| Entrez | 878955 |
| GenBank | AAG06867.1 |
| INSDC | AAG06867.1 |
| NCBI Locus Tag | PA3479 |
| protein_id(GenBank) | gb|AAG06867.1|AE004768_12|gnl|PseudoCAP|PA3479 |
| TIGR | NTL03PA03479 |
| UniParc | UPI0000133874 |
| UniProtKB Acc | Q51559 |
| UniProtKB ID | RHLA_PSEAE |
| UniRef100 | UniRef100_Q51559 |
| UniRef50 | UniRef50_Q51559 |
| UniRef90 | UniRef90_Q51559 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
rhamnosyltransferase chain A
|
| Synonyms | |
| Evidence for Translation | |
| Charge (pH 7) | 2.45 |
| Kyte-Doolittle Hydrophobicity Value | -0.180 |
| Molecular Weight (kDa) | 33.0 |
| Isoelectric Point (pI) | 7.46 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
Virulence Evidence
| Source 1 | |
| Database | PseudoCAP |
| Category | Rhamnolipid biosynthesis |
| Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
| Host Organism | Mus musculus |
| Notes | |
| Infection model | foreign-body Infection model |
| Infection model | pulmonary Infection model |
| Reference | 19594494 |
| Source 2 | |
| Database | PseudoCAP |
| Category | Rhamnolipid biosynthesis |
| Evidence |
ECO:0000315
mutant phenotype evidence used in manual assertion
|
| Host Organism | Homo sapiens |
| Notes | In vitro biofilms of P. aeruginosa PAO1 rhlA mutants yield fewer necrotic PMNs than wild-type PAO1. |
| Reference | 19594494 |
| Source 3 | |
| Database | VFDB : VF0089 |
| Category | Rhamnolipid biosynthesis |
| Evidence |
|
| Host Organism | |
| Notes | |
| Reference | |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 22 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG002163 (333 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA3479
Search term: rhlA
Search term: rhamnosyltransferase chain A
|
Human Homologs
References
|
RhlA converts beta-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the beta-hydroxydecanoyl-beta-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa.
Zhu K, Rock CO
J Bacteriol 2008 May;190(9):3147-54
PubMed ID: 18326581
|