Pseudomonas aeruginosa PAO1, PA3635 (eno)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3635
Name
eno
Replicon chromosome
Genomic location 4068677 - 4069966 (- strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_252325.1
GI 15598831
Affymetrix PA3635_eno_at
Entrez 880453
GenBank AAG07023.1
INSDC AAG07023.1
NCBI Locus Tag PA3635
protein_id(GenBank) gb|AAG07023.1|AE004783_8|gnl|PseudoCAP|PA3635
TIGR NTL03PA03635
UniParc UPI0000129F8A
UniProtKB Acc Q9HXZ5
UniProtKB ID ENO_PSEAE
UniRef100 UniRef100_A6V1F3
UniRef50 UniRef50_Q5XD01
UniRef90 UniRef90_Q88MF9

Product

Feature Type CDS
Coding Frame 1
Product Name
enolase
Product Name Confidence: Class 2
Synonyms 2-phosphoglycerate dehydratase
phosphopyruvate hydratase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -12.63
Kyte-Doolittle Hydrophobicity Value -0.142
Molecular Weight (kDa) 45.2
Isoelectric Point (pI) 4.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 663 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002039 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3635
Search term: eno
Search term: enolase

Human Homologs

Ensembl 110, assembly GRCh38.p14
enolase 3 [Source:HGNC Symbol;Acc:HGNC:3354]
E-value: 2.6e-118
Percent Identity: 54.1
Ensembl 110, assembly GRCh38.p14
enolase 3 [Source:HGNC Symbol;Acc:HGNC:3354]
E-value: 2.6e-118
Percent Identity: 54.1
Ensembl 110, assembly GRCh38.p14
enolase 3 [Source:HGNC Symbol;Acc:HGNC:3354]
E-value: 2.6e-118
Percent Identity: 54.1

References

ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt.
Thomson J, Gerstenberger PD, Goldberg DE, Gociar E, Orozco de Silva A, Fraenkel DG
J Bacteriol 1979 Jan;137(1):502-6
PubMed ID: 368027
A DEAD-box RNA helicase in the Escherichia coli RNA degradosome.
Py B, Higgins CF, Krisch HM, Carpousis AJ
Nature 1996 May 9;381(6578):169-72
PubMed ID: 8610017
Cloning, nucleotide sequence, and functional expression of the Escherichia coli enolase (eno) gene in a temperature-sensitive eno mutant strain.
Klein M, Sprenger GA, Freudl R
DNA Seq 1996;6(6):351-5
PubMed ID: 8988374