Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3859
|
Name |
|
Replicon | chromosome |
Genomic location | 4318259 - 4318906 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Optimal enzyme activity at 55 C and at pH 9.0 and is inhibited by phenylmethylsulfonyl fluoride. pI of 5.2 determined by IEF(theoretical pI of 5.0). Monomeric structure (24kDa). |
RefSeq | NP_252548.1 |
GI | 15599054 |
Affymetrix | PA3859_at |
Entrez | 879801 |
GenBank | AAG07246.1 |
INSDC | AAG07246.1 |
NCBI Locus Tag | PA3859 |
protein_id(GenBank) | gb|AAG07246.1|AE004803_2|gnl|PseudoCAP|PA3859 |
TIGR | NTL03PA03859 |
UniParc | UPI00000C5B47 |
UniProtKB Acc | Q9HXE7 |
UniProtKB ID | Q9HXE7_PSEAE |
UniRef100 | UniRef100_Q9HXE7 |
UniRef50 | UniRef50_Q53547 |
UniRef90 | UniRef90_Q9HXE7 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
carboxylesterase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -9.87 |
Kyte-Doolittle Hydrophobicity Value | 0.017 |
Molecular Weight (kDa) | 23.4 |
Isoelectric Point (pI) | 4.87 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
3CN7 | HYDROLASE | 03/25/08 | Crystal Structure Analysis of the Carboxylesterase PA3859 from Pseudomonas aeruginosa PAO1- MONOCLINIC CRYSTAL FORM | Pseudomonas aeruginosa | 2.99 | X-RAY DIFFRACTION | 100.0 |
3CN9 | HYDROLASE | 03/25/08 | Crystal Structure Analysis of the Carboxylesterase PA3859 from Pseudomonas aeruginosa PAO1- orthorhombic crystal form | Pseudomonas aeruginosa | 2.09 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 212 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG001810 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3859
Search term: carboxylesterase
|
Ensembl
110, assembly
GRCh38.p14
|
lysophospholipase 2 [Source:HGNC Symbol;Acc:HGNC:6738]
E-value:
1.3e-25
Percent Identity:
34.8
|
Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1.
Pesaresi A, Devescovi G, Lamba D, Venturi V, Degrassi G
Curr Microbiol 2005 Feb;50(2):102-9
PubMed ID: 15717224
|
Characterization of Pseudomonas fluorescens carboxylesterase: cloning and expression of the esterase gene in Escherichia coli.
Hong KH, Jang WH, Choi KD, Yoo OJ
Agric Biol Chem 1991 Nov;55(11):2839-45
PubMed ID: 1368750
|
Crystal structure of carboxylesterase from Pseudomonas fluorescens, an alpha/beta hydrolase with broad substrate specificity.
Kim KK, Song HK, Shin DH, Hwang KY, Choe S, Yoo OJ, Suh SW
Structure 1997 Dec 15;5(12):1571-84
PubMed ID: 9438866
|