Pseudomonas aeruginosa PAO1, PA3885 (tpbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3885
Name
tpbA
Replicon chromosome
Genomic location 4350834 - 4351490 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252574.1
GI 15599080
Affymetrix PA3885_at
DNASU PaCD00007691
Entrez 878776
GenBank AAG07272.1
INSDC AAG07272.1
NCBI Locus Tag PA3885
protein_id(GenBank) gb|AAG07272.1|AE004805_10|gnl|PseudoCAP|PA3885
TIGR NTL03PA03885
UniParc UPI00000C5B5A
UniProtKB Acc Q9HXC7
UniProtKB ID Q9HXC7_PSEAE
UniRef100 UniRef100_Q9HXC7
UniRef50 UniRef50_Q9HXC7
UniRef90 UniRef90_Q9HXC7

Product

Feature Type CDS
Coding Frame 1
Product Name
protein tyrosine phosphatase TpbA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 0.59
Kyte-Doolittle Hydrophobicity Value -0.111
Molecular Weight (kDa) 24.0
Isoelectric Point (pI) 7.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
cell fractionation evidence
Periplasmic Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4R0T HYDROLASE 08/01/14 Crystal structure of P. aeruginosa TpbA (C132S) in complex with pTyr Pseudomonas aeruginosa PAO1 2.603 X-RAY DIFFRACTION 99.5
4R0S HYDROLASE 08/01/14 Crystal structure of P. aeruginosa TpbA Pseudomonas aeruginosa PAO1 2.03 X-RAY DIFFRACTION 100.0
2M3V HYDROLASE 01/26/13 Solution structure of tyrosine phosphatase related to biofilm formation A (TpbA) from Pseudomonas aeruginosa Pseudomonas aeruginosa NOT SOLUTION NMR 97.4

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 32 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001786 (406 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3885
Search term: tpbA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Connecting quorum sensing, c-di-GMP, pel polysaccharide, and biofilm formation in Pseudomonas aeruginosa through tyrosine phosphatase TpbA (PA3885).
Ueda A, Wood TK
PLoS Pathog 2009 Jun;5(6):e1000483
PubMed ID: 19543378
Expression, purification, crystallization and preliminary crystallographic analysis of PA3885 (TpbA) from Pseudomonas aeruginosa PAO1.
Yang W, Li K, Bai Y, Zhou R, Zhou W, Bartlam M
Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Nov 1;66(Pt 11):1473-6
PubMed ID: 21045298