Pseudomonas aeruginosa PAO1, PA4245 (rpmD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4245
Name
rpmD
Replicon chromosome
Genomic location 4758891 - 4759067 (- strand)

Cross-References

RefSeq NP_252935.1
GI 15599441
Affymetrix PA4245_rpmD_at
DNASU PaCD00007842
Entrez 881787
GenBank AAG07633.1
INSDC AAG07633.1
NCBI Locus Tag PA4245
protein_id(GenBank) gb|AAG07633.1|AE004841_11|gnl|PseudoCAP|PA4245
TIGR NTL03PA04246
UniParc UPI00000C5C82
UniProtKB Acc Q9HWF3
UniProtKB ID RL30_PSEAE
UniRef100 UniRef100_A6UZK6
UniRef50 UniRef50_P44366
UniRef90 UniRef90_C1DKN1

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L30
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 6.43
Kyte-Doolittle Hydrophobicity Value -0.253
Molecular Weight (kDa) 6.5
Isoelectric Point (pI) 10.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 381 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001465 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4245
Search term: rpmD

Human Homologs

References

The primary structure of protein L30 from Escherichia coli ribosomes.
Ritter E, Wittmann-Liebold B
FEBS Lett 1975 Dec 1;60(1):153-5
PubMed ID: 776670
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M
Nucleic Acids Res 1983 May 11;11(9):2599-616
PubMed ID: 6222285
Structural comparison of the prokaryotic ribosomal proteins L7/L12 and L30.
Leijonmarck M, Appelt K, Badger J, Liljas A, Wilson KS, White SW
Proteins 1988;3(4):243-51
PubMed ID: 3047743