Pseudomonas aeruginosa PAO1, PA4247 (rplR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4247
Name
rplR
Replicon chromosome
Genomic location 4759574 - 4759924 (- strand)

Cross-References

RefSeq NP_252937.1
GI 15599443
Affymetrix PA4247_rplR_at
DNASU PaCD00007843
Entrez 881795
GenBank AAG07635.1
INSDC AAG07635.1
NCBI Locus Tag PA4247
protein_id(GenBank) gb|AAG07635.1|AE004841_13|gnl|PseudoCAP|PA4247
TIGR NTL03PA04248
UniParc UPI00000C5C83
UniProtKB Acc Q9HWF1
UniProtKB ID RL18_PSEAE
UniRef100 UniRef100_A6UZK4
UniRef50 UniRef50_Q9RSL2
UniRef90 UniRef90_C1DKM9

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L18
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 11.44
Kyte-Doolittle Hydrophobicity Value -0.384
Molecular Weight (kDa) 12.7
Isoelectric Point (pI) 10.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 590 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001463 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4247
Search term: rplR

Human Homologs

References

The primary structure of the 5S RNA binding protein L18 from Escherichia coli ribosomes.
Brosius J, Schiltz E, Chen R
FEBS Lett 1975 Aug 15;56(2):359-61
PubMed ID: 1098937
The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.
Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M
Nucleic Acids Res 1983 May 11;11(9):2599-616
PubMed ID: 6222285
Protein L18 binds primarily at the junctions of helix II and internal loops A and B in Escherichia coli 5 S RNA. Implications for 5 S RNA structure.
Egebjerg J, Christiansen J, Brown RS, Larsen N, Garrett RA
J Mol Biol 1989 Apr 20;206(4):651-68
PubMed ID: 2472486