Pseudomonas aeruginosa PAO1, PA4263 (rplC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4263
Name
rplC
Replicon chromosome
Genomic location 4766625 - 4767260 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252953.1
GI 15599459
Affymetrix PA4263_rplC_at
Entrez 881728
GenBank AAG07651.1
INSDC AAG07651.1
NCBI Locus Tag PA4263
protein_id(GenBank) gb|AAG07651.1|AE004841_29|gnl|PseudoCAP|PA4263
TIGR NTL03PA04264
UniParc UPI00000C5C8D
UniProtKB Acc Q9HWD5
UniProtKB ID RL3_PSEAE
UniRef100 UniRef100_A6UZI8
UniRef50 UniRef50_A2SLF7
UniRef90 UniRef90_Q889X1

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L3
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 9.90
Kyte-Doolittle Hydrophobicity Value -0.329
Molecular Weight (kDa) 22.6
Isoelectric Point (pI) 10.55

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 620 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001447 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4263
Search term: rplC

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L3 [Source:HGNC Symbol;Acc:HGNC:10379]
E-value: 1.1e-21
Percent Identity: 31.1
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L3 [Source:HGNC Symbol;Acc:HGNC:10379]
E-value: 1.1e-21
Percent Identity: 31.1
Ensembl 110, assembly GRCh38.p14
mitochondrial ribosomal protein L3 [Source:HGNC Symbol;Acc:HGNC:10379]
E-value: 1.1e-21
Percent Identity: 31.1

References

Regulation of the S10 ribosomal protein operon in E. coli: nucleotide sequence at the start of the operon.
Olins PO, Nomura M
Cell 1981 Oct;26(2 Pt 2):205-11
PubMed ID: 7037196
Structure of the Escherichia coli S10 ribosomal protein operon.
Zurawski G, Zurawski SM
Nucleic Acids Res 1985 Jun 25;13(12):4521-6
PubMed ID: 3892488
Interaction of the sarcin/ricin domain of 23 S ribosomal RNA with proteins L3 and L6.
Uchiumi T, Sato N, Wada A, Hachimori A
J Biol Chem 1999 Jan 8;274(2):681-6
PubMed ID: 9873002
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142