Pseudomonas aeruginosa PAO1, PA4708 (phuT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4708
Name
phuT
Synonym: phuT
Replicon chromosome
Genomic location 5287069 - 5287962 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253396.1
GI 15599902
Affymetrix PA4708_at
DNASU PaCD00008043
Entrez 881543
GenBank AAG08094.1
INSDC AAG08094.1
NCBI Locus Tag PA4708
protein_id(GenBank) gb|AAG08094.1|AE004884_12|gnl|PseudoCAP|PA4708
TIGR NTL03PA04709
UniParc UPI00000C5DD6
UniProtKB Acc Q9HV90
UniProtKB ID Q9HV90_PSEAE
UniRef100 UniRef100_W0YZR5
UniRef50 UniRef50_O68879
UniRef90 UniRef90_O68879

Product

Feature Type CDS
Coding Frame 1
Product Name
Heme-transport protein, PhuT
Product Name Confidence: Class 2
Synonyms Heme-transport protein, PhuT
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.72
Kyte-Doolittle Hydrophobicity Value 0.016
Molecular Weight (kDa) 31.0
Isoelectric Point (pI) 7.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2R79 TRANSPORT PROTEIN 09/07/07 Crystal Structure of a Periplasmic Heme Binding Protein from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.4 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 309 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004355 (463 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4708
Search term: phuT

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Transport of haemin across the cytoplasmic membrane through a haemin-specific periplasmic binding-protein-dependent transport system in Yersinia enterocolitica.
Stojiljkovic I, Hantke K
Mol Microbiol 1994 Aug;13(4):719-32
PubMed ID: 7997183
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
Ochsner UA, Vasil ML
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):4409-14
PubMed ID: 8633080
Cloning and characterization of a novel periplasmic heme-transport protein from the human pathogen Pseudomonas aeruginosa.
Tong Y, Guo M
J Biol Inorg Chem 2007 Aug;12(6):735-50
PubMed ID: 17387526
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994