Pseudomonas aeruginosa PAO1, PA4956 (rhdA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4956
Name
rhdA
Replicon chromosome
Genomic location 5561599 - 5562414 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Commentinvolvement in cyanide detoxification

Cross-References

RefSeq NP_253643.1
GI 15600149
Affymetrix PA4956_rhdA_at
DNASU PaCD00008142
Entrez 879172
GenBank AAG08341.1
INSDC AAG08341.1
NCBI Locus Tag PA4956
protein_id(GenBank) gb|AAG08341.1|AE004908_7|gnl|PseudoCAP|PA4956
TIGR NTL03PA04957
UniParc UPI00000C5E93
UniProtKB Acc Q9HUK9
UniProtKB ID Q9HUK9_PSEAE
UniRef100 UniRef100_Q9HUK9
UniRef50 UniRef50_Q9HUK9
UniRef90 UniRef90_Q9HUK9

Product

Feature Type CDS
Coding Frame 1
Product Name
thiosulfate:cyanide sulfurtransferase
Product Name Confidence: Class 1
Synonyms rhodanese-like protein
thiosulfate:cyanide sulfurtransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.15
Kyte-Doolittle Hydrophobicity Value -0.190
Molecular Weight (kDa) 29.4
Isoelectric Point (pI) 5.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 385 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004571 (517 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4956
Search term: rhdA

Human Homologs

References

Cloning, sequence analysis and overexpression of the rhodanese gene of Azotobacter vinelandii.
Colnaghi R, Pagani S, Kennedy C, Drummond M
Eur J Biochem 1996 Feb 15;236(1):240-8
PubMed ID: 8617271
Involvement of Pseudomonas aeruginosa rhodanese in protection from cyanide toxicity.
Cipollone R, Frangipani E, Tiburzi F, Imperi F, Ascenzi P, Visca P
Appl Environ Microbiol 2007 Jan;73(2):390-8
PubMed ID: 17098912