Pseudomonas aeruginosa PAO1, PA5100 (hutU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5100
Name
hutU
Replicon chromosome
Genomic location 5743074 - 5744753 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253787.1
GI 15600293
Affymetrix PA5100_hutU_at
Entrez 880890
GenBank AAG08485.1
INSDC AAG08485.1
NCBI Locus Tag PA5100
protein_id(GenBank) gb|AAG08485.1|AE004923_1|gnl|PseudoCAP|PA5100
TIGR NTL03PA05101
UniParc UPI000012CEC4
UniProtKB Acc Q9HU83
UniProtKB ID HUTU_PSEAE
UniRef100 UniRef100_Q9HU83
UniRef50 UniRef50_P25080
UniRef90 UniRef90_Q9HU83

Product

Feature Type CDS
Coding Frame 1
Product Name
urocanase
Product Name Confidence: Class 2
Synonyms urocanate hydratase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.07
Kyte-Doolittle Hydrophobicity Value -0.244
Molecular Weight (kDa) 61.2
Isoelectric Point (pI) 6.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1UWL HYDROLASE 02/05/04 1.76A Structure of Urocanate Hydratase from Pseudomonas putida PSEUDOMONAS PUTIDA 1.76 X-RAY DIFFRACTION 91.4
1W1U HYDROLASE 06/24/04 Inactive Urocanase-SA cocrystallized with urocanate PSEUDOMONAS PUTIDA 2.23 X-RAY DIFFRACTION 91.2
1UWK HYDROLASE 02/05/04 The High Resolution Structure of Urocanate Hydratase from Pseudomonas putida in complex with urocanate PSEUDOMONAS PUTIDA 1.19 X-RAY DIFFRACTION 91.2
2V7G LYASE 07/30/07 Crystal Structure of an Engineered Urocanase Tetramer PSEUDOMONAS PUTIDA 2 X-RAY DIFFRACTION 91.2

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 266 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004696 (504 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5100
Search term: hutU
Search term: urocanase

Human Homologs

Ensembl 110, assembly GRCh38.p14
urocanate hydratase 1 [Source:HGNC Symbol;Acc:HGNC:26444]
E-value: 5.5e-84
Percent Identity: 33.4

References

Cloning, expression and mutational analysis of the urocanase gene (hutU) from Pseudomonas putida.
Lenz M, Rétey J
Eur J Biochem 1993 Oct 1;217(1):429-34
PubMed ID: 7901006