Pseudomonas aeruginosa PAO1, PA5239 (rho)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5239
Name
rho
Replicon chromosome
Genomic location 5898865 - 5900124 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253926.1
GI 15600432
Affymetrix PA5239_rho_at
DNASU PaCD00008256
Entrez 880855
GenBank AAG08624.1
INSDC AAG08624.1
NCBI Locus Tag PA5239
protein_id(GenBank) gb|AAG08624.1|AE004936_7|gnl|PseudoCAP|PA5239
TIGR NTL03PA05240
UniParc UPI00000C5F6F
UniProtKB Acc Q9HTV1
UniProtKB ID RHO_PSEAE
UniRef100 UniRef100_Q9HTV1
UniRef50 UniRef50_Q9HTV1
UniRef90 UniRef90_Q9HTV1

Product

Feature Type CDS
Coding Frame 1
Product Name
transcription termination factor Rho
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.58
Kyte-Doolittle Hydrophobicity Value -0.364
Molecular Weight (kDa) 47.1
Isoelectric Point (pI) 6.82

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 544 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004819 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5239
Search term: rho

Human Homologs

References

Site-directed alterations in the ATP-binding domain of rho protein affect its activities as a termination factor.
Dombroski AJ, Brennan CA, Spear P, Platt T
J Biol Chem 1988 Dec 15;263(35):18802-9
PubMed ID: 2461932
Phylogenetic analysis of sequences from diverse bacteria with homology to the Escherichia coli rho gene.
Opperman T, Richardson JP
J Bacteriol 1994 Aug;176(16):5033-43
PubMed ID: 8051015
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994