Pseudomonas aeruginosa PAO1, PA0082 (tssA1)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 100124 - 101158 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1


RefSeq NP_248772.1
GI 15595280
Affymetrix PA0082_at
DNASU PaCD00006035
Entrez 879402
GenBank AAG03472.1
INSDC AAG03472.1
NCBI Locus Tag PA0082
protein_id(GenBank) gb|AAG03472.1|AE004447_5|gnl|PseudoCAP|PA0082
UniParc UPI00000C4F23
UniProtKB Acc Q9I750
UniRef100 UniRef100_Q9I750
UniRef50 UniRef50_Q4K3N9
UniRef90 UniRef90_Q9I750


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -23.53
Kyte-Doolittle Hydrophobicity Value 0.010
Molecular Weight (kDa) 36.6
Isoelectric Point (pI) 4.14

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Reference 16763151
Source 2
Database Victors : 4228
Category Not available / Not known
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Rattus norvegicus
Infection model Rat agar bead model of chronic lung infection
Reference 12023080

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 81 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000081 (1226 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0082
Search term: tssA1
Search term: TssA1

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 41814
Genomic Position 100432
Position in ORF 309(1035)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 48921
Genomic Position 100443
Position in ORF 320(1035)
Reference 14617778

Transposon Insertions in Orthologs


A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433
TssA forms a gp6-like ring attached to the type VI secretion sheath.
Planamente S, Salih O, Manoli E, Albesa-Jové D, Freemont PS, Filloux A
EMBO J. 2016 Aug;35(15):1613-27
PubMed ID: 27288401