Pseudomonas aeruginosa PAO1, PA0083 (tssB1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0083
Name
tssB1
Replicon chromosome
Genomic location 101247 - 101765 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248773.1
GI 15595281
Affymetrix PA0083_at
DNASU PaCD00006036
Entrez 879476
GenBank AAG03473.1
INSDC AAG03473.1
NCBI Locus Tag PA0083
protein_id(GenBank) gb|AAG03473.1|AE004447_6|gnl|PseudoCAP|PA0083
TIGR NTL03PA00084
UniParc UPI00000C4F24
UniProtKB Acc Q9I749
UniProtKB ID Q9I749_PSEAE
UniRef100 UniRef100_W0YKW6
UniRef50 UniRef50_J2YIR2
UniRef90 UniRef90_J2YIR2

Product

Feature Type CDS
Coding Frame 1
Product Name
TssB1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:4.7, molecular weight:16.8, PMID:21674802)
Detected by two-dimensional gel electrophoresis, mass spectral analysis and protein mass mapping (PMID:21863142).
Charge (pH 7) -3.00
Kyte-Doolittle Hydrophobicity Value -0.251
Molecular Weight (kDa) 18.8
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5N8N STRUCTURAL PROTEIN 02/23/17 Contracted sheath of a Pseudomonas aeruginosa type six secretion system consisting of TssB1 and TssC1 PSEUDOMONAS AERUGINOSA 3.28 ELECTRON MICROSCOPY 100.0
4UQZ PROTEIN TRANSPORT 06/25/14 Coevolution of the ATPase ClpV, the TssB-TssC Sheath and the Accessory HsiE Protein Distinguishes Two Type VI Secretion Classes PSEUDOMONAS AERUGINOSA PAO1 1.599 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 107 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000082 (1260 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0083
Search term: tssB1
Search term: TssB1

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading, Engl.) 2007 Aug;153(Pt 8):2689-99
PubMed ID: 17660433
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802