Pseudomonas aeruginosa PAO1, PA0169 (siaD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0169
Name
siaD
Replicon chromosome
Genomic location 192965 - 193672 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248859.1
GI 15595367
Affymetrix PA0169_at
Entrez 879346
GenBank AAG03559.1
INSDC AAG03559.1
NCBI Locus Tag PA0169
protein_id(GenBank) gb|AAG03559.1|AE004454_11|gnl|PseudoCAP|PA0169
TIGR NTL03PA00170
UniParc UPI00000C4F78
UniProtKB Acc Q9I6W3
UniProtKB ID Q9I6W3_PSEAE
UniRef100 UniRef100_Q9I6W3
UniRef50 UniRef50_Q9I6W3
UniRef90 UniRef90_Q9I6W3

Product

Feature Type CDS
Coding Frame 1
Product Name
SiaD
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.91
Kyte-Doolittle Hydrophobicity Value -0.539
Molecular Weight (kDa) 26.8
Isoelectric Point (pI) 9.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 454 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000167 (2894 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0169
Search term: siaD
Search term: SiaD

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 43719
Genomic Position 193288
Position in ORF 324(708)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_02110
Mutant ID PA14NR: 55715
Genomic Position 192451
Position in ORF 627(708)
Reference 16477005

References

Detergent-induced cell aggregation in subpopulations of Pseudomonas aeruginosa as a preadaptive survival strategy.
Klebensberger J, Lautenschlager K, Bressler D, Wingender J, Philipp B.
Environ Microbiol. 2007 Sep;9(9):2247-59.
PubMed ID: 17686022
SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa.
Klebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B.
Environ Microbiol. 2009 Dec;11(12):3073-86.
PubMed ID: 19638175
SiaA/D Interconnects c-di-GMP and RsmA Signaling to Coordinate Cellular Aggregation of Pseudomonas aeruginosa in Response to Environmental Conditions.
Colley B(1), Dederer V(2), Carnell M(3), Kjelleberg S(4), Rice SA(4), Klebensberger J(2).
Front Microbiol. 2016 Feb 26;7:179.
PubMed ID: 26955366