Pseudomonas aeruginosa PAO1, PA0342 (thyA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0342
Name
thyA
Replicon chromosome
Genomic location 384733 - 385527 (+ strand)

Cross-References

RefSeq NP_249033.1
GI 15595539
Affymetrix PA0342_thyA_at
DNASU PaCD00006152
Entrez 879417
GenBank AAG03731.1
INSDC AAG03731.1
NCBI Locus Tag PA0342
protein_id(GenBank) gb|AAG03731.1|AE004472_3|gnl|PseudoCAP|PA0342
TIGR NTL03PA00343
UniParc UPI00001378E8
UniProtKB Acc Q9I6F1
UniProtKB ID TYSY_PSEAE
UniRef100 UniRef100_B7V2Q5
UniRef50 UniRef50_Q12CI6
UniRef90 UniRef90_A6UYE6

Product

Feature Type CDS
Coding Frame 1
Product Name
thymidylate synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.24
Kyte-Doolittle Hydrophobicity Value -0.154
Molecular Weight (kDa) 30.0
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 401 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000334 (406 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0342
Search term: thyA

Human Homologs

Ensembl 97, assembly GRCh38.p12
thymidylate synthetase [Source:HGNC Symbol;Acc:HGNC:12441]
E-value: 3.4e-80
Percent Identity: 51.4

References

Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.
Belfort M, Maley G, Pedersen-Lane J, Maley F
Proc Natl Acad Sci U S A 1983 Aug;80(16):4914-8
PubMed ID: 6308660
Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA.
Voeller DM, Changchien LM, Maley GF, Maley F, Takechi T, Turner RE, Montfort WR, Allegra CJ, Chu E
Nucleic Acids Res. 1995 Mar;23(5):869-75
PubMed ID: 7708505