Pseudomonas aeruginosa PAO1, PA0518 (nirM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0518
Name
nirM
Replicon chromosome
Genomic location 578029 - 578343 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249209.1
GI 15595715
Affymetrix PA0518_nirM_at
DNASU PaCD00006218
Entrez 882220
GenBank AAG03907.1
INSDC AAG03907.1
NCBI Locus Tag PA0518
protein_id(GenBank) gb|AAG03907.1|AE004488_11|gnl|PseudoCAP|PA0518
TIGR NTL03PA00519
UniParc UPI0000126C4A
UniProtKB Acc P00099
UniProtKB ID CY551_PSEAE
UniRef100 UniRef100_P00099
UniRef50 UniRef50_P95339
UniRef90 UniRef90_P00099

Product

Feature Type CDS
Coding Frame 1
Product Name
cytochrome c-551 precursor
Product Name Confidence: Class 1
Synonyms cytochrome c8
c551
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.16
Kyte-Doolittle Hydrophobicity Value -0.056
Molecular Weight (kDa) 11.0
Isoelectric Point (pI) 7.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
351C ELECTRON TRANSPORT 07/20/81 STRUCTURE OF CYTOCHROME C551 FROM P. AERUGINOSA REFINED AT 1.6 ANGSTROMS RESOLUTION AND COMPARISON OF THE TWO REDOX FORMS PSEUDOMONAS AERUGINOSA 1.6 X-RAY DIFFRACTION 100.0
1DVV ELECTRON TRANSPORT 01/22/00 SOLUTION STRUCTURE OF THE QUINTUPLE MUTANT OF CYTOCHROME C-551 FROM PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 93.9
2PAC ELECTRON TRANSPORT 05/05/93 SOLUTION STRUCTURE OF FE(II) CYTOCHROME C551 FROM PSEUDOMONAS AERUGINOSA AS DETERMINED BY TWO-DIMENSIONAL 1H NMR PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0
2EXV ELECTRON TRANSPORT 11/09/05 Crystal structure of the F7A mutant of the cytochrome c551 from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.86 X-RAY DIFFRACTION 98.8
3X39 ELECTRON TRANSPORT 01/16/15 Domain-swapped dimer of Pseudomonas aeruginosa cytochrome c551 PSEUDOMONAS AERUGINOSA PAO1 1.5 X-RAY DIFFRACTION 100.0
451C ELECTRON TRANSPORT 07/20/81 STRUCTURE OF CYTOCHROME C551 FROM P. AERUGINOSA REFINED AT 1.6 ANGSTROMS RESOLUTION AND COMPARISON OF THE TWO REDOX FORMS PSEUDOMONAS AERUGINOSA 1.6 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 88 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000502 (380 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0518
Search term: nirM

References

The structural gene for cytochrome c551 from Pseudomonas aeruginosa. The nucleotide sequence shows a location downstream of the nitrite reductase gene.
Nordling M, Young S, Karlsson BG, Lundberg LG
FEBS Lett. 1990 Jan;259(2):230-2
PubMed ID: 2152881
Investigation of the structure of oxidized Pseudomonas aeruginosa cytochrome c-551 by NMR: comparison of observed paramagnetic shifts and calculated pseudocontact shifts.
Timkovich R, Cai M
Biochemistry 1993 Nov;32(43):11516-23
PubMed ID: 8218218