Pseudomonas aeruginosa PAO1, PA0896 (aruF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0896
Name
aruF
Synonym: aruAI
Replicon chromosome
Genomic location 979329 - 980345 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249587.1
GI 15596093
Affymetrix PA0896_aruF_at
DNASU PaCD00006413
Entrez 879437
GenBank AAG04285.1
INSDC AAG04285.1
NCBI Locus Tag PA0896
protein_id(GenBank) gb|AAG04285.1|AE004524_2|gnl|PseudoCAP|PA0896
TIGR NTL03PA00897
UniParc UPI00001261AE
UniProtKB Acc P80357
UniProtKB ID ASTA_PSEAE
UniRef100 UniRef100_P80357
UniRef50 UniRef50_P80357
UniRef90 UniRef90_P80357

Product

Feature Type CDS
Coding Frame 1
Product Name
subunit I of arginine N2-succinyltransferase = ornithine N2-succinyltransferase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.13
Kyte-Doolittle Hydrophobicity Value 0.003
Molecular Weight (kDa) 36.9
Isoelectric Point (pI) 4.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1YLE TRANSFERASE 01/19/05 The structure of arginine/ornithine succinyltransferase subunit AI from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 75 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000863 (547 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0896
Search term: aruF

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 36821
Genomic Position 980015
Position in ORF 687(1017)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 50121
Genomic Position 979897
Position in ORF 569(1017)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_52700
Mutant ID PA14NR: 56181
Genomic Position 4672104
Position in ORF 859(1017)
Reference 16477005

References

Cloning and characterization of argR, a gene that participates in regulation of arginine biosynthesis and catabolism in Pseudomonas aeruginosa PAO1.
Park SM, Lu CD, Abdelal AT.
J Bacteriol. 1997 Sep;179(17):5300-8.
PubMed ID: 9286980
Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa.
Itoh Y.
J Bacteriol. 1997 Dec;179(23):7280-90.
PubMed ID: 9393691
N-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa.
Jann A(1), Stalon V, Wauven CV, Leisinger T, Haas D.
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4937-41.
PubMed ID: 16593724