Pseudomonas aeruginosa PAO1, PA0907 (alpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0907
Name
alpA
Replicon chromosome
Genomic location 992714 - 993244 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_249598.1
GI 15596104
Affymetrix PA0907_at
DNASU PaCD00006418
Entrez 878349
GenBank AAG04296.1
INSDC AAG04296.1
NCBI Locus Tag PA0907
protein_id(GenBank) gb|AAG04296.1|AE004525_4|gnl|PseudoCAP|PA0907
TIGR NTL03PA00908
UniParc UPI00000C51AE
UniProtKB Acc Q9I550
UniProtKB ID Q9I550_PSEAE
UniRef100 UniRef100_W0YMZ6
UniRef50 UniRef50_W0YMZ6
UniRef90 UniRef90_W0YMZ6

Product

Feature Type CDS
Coding Frame 1
Product Name
lysis phenotype activator, AlpA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.20
Kyte-Doolittle Hydrophobicity Value -0.290
Molecular Weight (kDa) 20.2
Isoelectric Point (pI) 10.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 3 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000873 (308 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0907
Search term: alpA

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 39835
Genomic Position 993030
Position in ORF 317(531)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 50463
Genomic Position 992740
Position in ORF 27(531)
Reference 14617778

Transposon Insertions in Orthologs

References

A self-lysis pathway that enhances the virulence of a pathogenic bacterium.
McFarland KA(1), Dolben EL(2), LeRoux M(3), Kambara TK(1), Ramsey KM(1), Kirkpatrick RL(4), Mougous JD(4), Hogan DA(2), Dove SL(5).
Proc Natl Acad Sci U S A. 2015 Jul 7;112(27):8433-8.
PubMed ID: 26100878