Pseudomonas aeruginosa PAO1, PA1252 (dpkA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1252
Name
dpkA
Replicon chromosome
Genomic location 1359590 - 1360594 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249943.1
GI 15596449
Affymetrix PA1252_at
DNASU PaCD00006578
Entrez 881290
GenBank AAG04641.1
INSDC AAG04641.1
NCBI Locus Tag PA1252
protein_id(GenBank) gb|AAG04641.1|AE004555_1|gnl|PseudoCAP|PA1252
TIGR NTL03PA01253
UniParc UPI000013A4EA
UniProtKB Acc Q9I492
UniProtKB ID PY2CR_PSEAE
UniRef100 UniRef100_Q9I492
UniRef50 UniRef50_Q9I492
UniRef90 UniRef90_Q9I492

Product

Feature Type CDS
Coding Frame 1
Product Name
DpkA
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -7.22
Kyte-Doolittle Hydrophobicity Value 0.013
Molecular Weight (kDa) 35.7
Isoelectric Point (pI) 5.93

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 206 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001204 (520 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1252
Search term: dpkA
Search term: DpkA

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 53073
Genomic Position 1360324
Position in ORF 735(1005)
Reference 14617778
Type ISlacZ/hah insertion site
Mutant ID UWGC: 15620
Genomic Position 1360462
Position in ORF 873(1005)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 53969
Genomic Position 1360324
Position in ORF 735(1005)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_48020
Mutant ID PA14NR: 43118
Genomic Position 4274866
Position in ORF 866(1005)
Reference 16477005

References

Properties and primary structure of the L-malate dehydrogenase from the extremely thermophilic archaebacterium Methanothermus fervidus.
Honka E, Fabry S, Niermann T, Palm P, Hensel R.
Eur J Biochem. 1990 Mar 30;188(3):623-32.
PubMed ID: 2110059
Molecular characterization of LhpR in control of hydroxyproline catabolism and transport in Pseudomonas aeruginosa PAO1.
Li G(1), Lu CD(2).
Microbiology. 2016 Jul;162(7):1232-42.
PubMed ID: 27145750