Pseudomonas aeruginosa PAO1, PA1409 (aphA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1409
Name
aphA
Replicon chromosome
Genomic location 1533238 - 1534278 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250100.1
GI 15596606
Affymetrix PA1409_aphA_at
DNASU PaCD00006650
Entrez 879295
GenBank AAG04798.1
INSDC AAG04798.1
NCBI Locus Tag PA1409
protein_id(GenBank) gb|AAG04798.1|AE004570_6|gnl|PseudoCAP|PA1409
TIGR NTL03PA01410
UniParc UPI00000C5366
UniProtKB Acc Q9I3T5
UniProtKB ID Q9I3T5_PSEAE
UniRef100 UniRef100_Q9I3T5
UniRef50 UniRef50_Q9I3T5
UniRef90 UniRef90_Q9I3T5

Product

Feature Type CDS
Coding Frame 1
Product Name
acetylpolyamine aminohydrolase
Product Name Confidence: Class 1
Evidence for Translation
Charge (pH 7) -12.27
Kyte-Doolittle Hydrophobicity Value -0.231
Molecular Weight (kDa) 38.0
Isoelectric Point (pI) 5.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 438 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001330 (513 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1409
Search term: aphA

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 33943
Genomic Position 1534122
Position in ORF 885(1041)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_46230
Mutant ID PA14NR: 53313
Genomic Position 4110588
Position in ORF 650(1041)
Reference 16477005

References

Crystallization and some properties of acetylpolyamine amidohydrolase from Mycoplana bullata.
Fujishiro K, Ando M, Uwajima T.
Biochem Biophys Res Commun. 1988 Dec 30;157(3):1169-74.
PubMed ID: 3207420
Acetylpolyamine amidohydrolase from Mycoplana ramosa: gene cloning and characterization of the metal-substituted enzyme.
Sakurada K, Ohta T, Fujishiro K, Hasegawa M, Aisaka K.
J Bacteriol. 1996 Oct;178(19):5781-6.
PubMed ID: 8824626
Transcriptome analysis of agmatine and putrescine catabolism in Pseudomonas aeruginosa PAO1.
Chou HT, Kwon DH, Hegazy M, Lu CD.
J Bacteriol. 2008 Mar;190(6):1966-75.
PubMed ID: 18192388
A fluorescence lifetime-based binding assay for acetylpolyamine amidohydrolases from Pseudomonas aeruginosa using a [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) ligand probe.
Meyners C(1), Wawrzinek R, Krämer A, Hinz S, Wessig P, Meyer-Almes FJ.
Anal Bioanal Chem. 2014 Aug;406(20):4889-97.
PubMed ID: 24871864
Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa.
Krämer A(1), Herzer J(2), Overhage J(2), Meyer-Almes FJ(3).
BMC Biochem. 2016 Mar 9;17:4.
PubMed ID: 26956223