Pseudomonas aeruginosa PAO1, PA1706 (pcrV)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1706
Name
pcrV
Replicon chromosome
Genomic location 1852288 - 1853172 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250397.1
GI 15596903
Affymetrix PA1706_pcrV_at
Entrez 882997
GenBank AAG05095.1
INSDC AAG05095.1
NCBI Locus Tag PA1706
protein_id(GenBank) gb|AAG05095.1|AE004597_9|gnl|PseudoCAP|PA1706
TIGR NTL03PA01707
UniParc UPI00000D424F
UniProtKB Acc G3XD49
UniProtKB ID G3XD49_PSEAE
UniRef100 UniRef100_W0YNT8
UniRef50 UniRef50_Q02KJ7
UniRef90 UniRef90_Q02KJ7

Product

Feature Type CDS
Coding Frame 1
Product Name
type III secretion protein PcrV
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -7.51
Kyte-Doolittle Hydrophobicity Value -0.257
Molecular Weight (kDa) 32.3
Isoelectric Point (pI) 4.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6CYF IMMUNE SYSTEM 04/05/18 PcrV fragment with bound Fab homo sapiens; Pseudomonas aeruginosa 2.78 X-RAY DIFFRACTION 96.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
KB001 DB05222 DrugBank 5.1.4 O30527 Low calcium response locus protein V 100.0 294 5.4e-158

Virulence Evidence

Source 1
Database VFDB : VF0083
Category T3SS
Evidence
Host Organism
Notes
Reference 11372029

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 5 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003683 (315 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1706
Search term: pcrV

Human Homologs

References

LcrV is a channel size-determining component of the Yop effector translocon of Yersinia.
Holmström A, Olsson J, Cherepanov P, Maier E, Nordfelth R, Pettersson J, Benz R, Wolf-Watz H, Forsberg A
Mol Microbiol 2001 Feb;39(3):620-32
PubMed ID: 11169103
Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon.
Yahr TL, Mende-Mueller LM, Friese MB, Frank DW
J Bacteriol 1997 Nov;179(22):7165-8
PubMed ID: 9371466
Active and passive immunization with the Pseudomonas V antigen protects against type III intoxication and lung injury.
Sawa T, Yahr TL, Ohara M, Kurahashi K, Gropper MA, Wiener-Kronish JP, Frank DW
Nat Med 1999 Apr;5(4):392-8
PubMed ID: 10202927
The V antigen of Pseudomonas aeruginosa is required for assembly of the functional PopB/PopD translocation pore in host cell membranes.
Goure J, Pastor A, Faudry E, Chabert J, Dessen A, Attree I
Infect Immun 2004 Aug;72(8):4741-50
PubMed ID: 15271936
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J Bacteriol 2015 Feb 15;197(4):762-73
PubMed ID: 25488299