Pseudomonas aeruginosa PAO1, PA1714 (exsD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1714
Name
exsD
Replicon chromosome
Genomic location 1858207 - 1859037 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentExsD inhibits transcription of the type III secretion system by binding to and inhibiting ExsA-dependent transcription. ExsD is classified as an anti-activator.

Cross-References

RefSeq NP_250405.1
GI 15596911
Affymetrix PA1714_at
DNASU PaCD00006776
Entrez 880880
GenBank AAG05103.1
INSDC AAG05103.1
NCBI Locus Tag PA1714
protein_id(GenBank) gb|AAG05103.1|AE004598_2|gnl|PseudoCAP|PA1714
TIGR NTL03PA01715
UniParc UPI00000C5459
UniProtKB Acc Q9I321
UniProtKB ID Q9I321_PSEAE
UniRef100 UniRef100_W0YMR8
UniRef50 UniRef50_P95429
UniRef90 UniRef90_P95429

Product

Feature Type CDS
Coding Frame 1
Product Name
ExsD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.72
Kyte-Doolittle Hydrophobicity Value -0.333
Molecular Weight (kDa) 31.4
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3FD9 UNKNOWN FUNCTION 11/25/08 Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa Pseudomonas aeruginosa UCBPP-PA14 2.6 X-RAY DIFFRACTION 99.2

Virulence Evidence

Source 1
Database VFDB : VF0083
Category T3SS
Evidence
Host Organism
Notes
Reference 11372029

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 5 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003675 (309 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1714
Search term: exsD
Search term: ExsD

Human Homologs

References

ExsD is a negative regulator of the Pseudomonas aeruginosa type III secretion regulon.
McCaw ML, Lykken GL, Singh PK, Yahr TL
Mol Microbiol 2002 Nov;46(4):1123-33
PubMed ID: 12421316
Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway.
Yahr TL, Goranson J, Frank DW
Mol Microbiol 1996 Dec;22(5):991-1003
PubMed ID: 8971719