Pseudomonas aeruginosa PAO1, PA1718 (pscE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1718
Name
pscE
Replicon chromosome
Genomic location 1862558 - 1862761 (+ strand)
CommentThe cytoplasmic PscE and PscG chaperones prevent PscF from premature polymerization in the cytoplasm.

Cross-References

RefSeq NP_250409.1
GI 15596915
Affymetrix PA1718_pscE_at
DNASU PaCD00006778
Entrez 881908
GenBank AAG05107.1
INSDC AAG05107.1
NCBI Locus Tag PA1718
protein_id(GenBank) gb|AAG05107.1|AE004598_6|gnl|PseudoCAP|PA1718
TIGR NTL03PA01719
UniParc UPI00000C545D
UniProtKB Acc Q9I317
UniProtKB ID PSCE_PSEAE
UniRef100 UniRef100_Q9I317
UniRef50 UniRef50_Q9I317
UniRef90 UniRef90_Q9I317

Product

Feature Type CDS
Coding Frame 1
Product Name
type III export protein PscE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.60
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 7.3
Isoelectric Point (pI) 6.50

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2UWJ CHAPERONE 03/22/07 Structure of the heterotrimeric complex which regulates type III secretion needle formation PSEUDOMONAS AERUGINOSA 2 X-RAY DIFFRACTION 98.5

Virulence Evidence

Source 1
Database VFDB : VF0083
Category T3SS
Evidence
Host Organism
Notes
Reference 11372029

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003671 (293 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1718
Search term: pscE

Human Homologs

References

The PscE-PscF-PscG complex controls type III secretion needle biogenesis in Pseudomonas aeruginosa.
Quinaud M, Chabert J, Faudry E, Neumann E, Lemaire D, Pastor A, Elsen S, Dessen A, Attree I
J Biol Chem 2005 Oct 28;280(43):36293-300
PubMed ID: 16115870
Analysis of virC, an operon involved in the secretion of Yop proteins by Yersinia enterocolitica.
Michiels T, Vanooteghem JC, Lambert de Rouvroit C, China B, Gustin A, Boudry P, Cornelis GR
J Bacteriol 1991 Aug;173(16):4994-5009
PubMed ID: 1860816
Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway.
Yahr TL, Goranson J, Frank DW
Mol Microbiol 1996 Dec;22(5):991-1003
PubMed ID: 8971719