Pseudomonas aeruginosa PAO1, PA1786 (nasS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1786
Name
nasS
Synonym: nasS
Synonym: nasF
Replicon chromosome
Genomic location 1933649 - 1934857 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250477.1
GI 15596983
Affymetrix PA1786_at
Entrez 879971
GenBank AAG05175.1
INSDC AAG05175.1
NCBI Locus Tag PA1786
protein_id(GenBank) gb|AAG05175.1|AE004604_5|gnl|PseudoCAP|PA1786
TIGR NTL03PA01787
UniParc UPI00000C5499
UniProtKB Acc Q9I2V6
UniProtKB ID Q9I2V6_PSEAE
UniRef100 UniRef100_Q9I2V6
UniRef50 UniRef50_V5Z8B1
UniRef90 UniRef90_B7VB93

Product

Feature Type CDS
Coding Frame 1
Product Name
NasS
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis (PMID:21863142).
Charge (pH 7) -2.96
Kyte-Doolittle Hydrophobicity Value -0.128
Molecular Weight (kDa) 43.6
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 204 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003179 (893 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1786
Search term: nasS
Search term: NasS

Human Homologs

References

NasFED proteins mediate assimilatory nitrate and nitrite transport in Klebsiella oxytoca (pneumoniae) M5al.
Wu Q, Stewart V
J Bacteriol 1998 Mar;180(5):1311-22
PubMed ID: 9495773
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Transcriptional regulation of nitrate assimilation in Pseudomonas aeruginosa occurs via transcriptional antitermination within the nirBD-PA1779-cobA operon.
Romeo A, Sonnleitner E, Sorger-Domenigg T, Nakano M, Eisenhaber B, Bläsi U
Microbiology (Reading) 2012 Jun;158(Pt 6):1543-1552
PubMed ID: 22493305