Pseudomonas aeruginosa PAO1, PA1810 (nppA2)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA1810
Name	nppA2 Synonym: oppB
Replicon	chromosome
Genomic location	1965771 - 1967618 (- strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	3 transposon mutants in orthologs
Comment	Structural similarity to 4ONY (ABC transporter from Brucella melitensis da Thermotoga maritima, root-mean-square deviation:0.3 angstroms). Sequence similarity to 4ONY (96%/42,44%) (no ligand). Final predicted ligand: No Ligand (PMID: 31631799)

Cross-References

RefSeq	NP_250501.1
GI	15597007
Affymetrix	PA1810_at
Entrez	880658
GenBank	AAG05199.1
INSDC	AAG05199.1
NCBI Locus Tag	PA1810
protein_id(GenBank)	gb|AAG05199.1|AE004607_5|gnl|PseudoCAP|PA1810
TIGR	NTL03PA01811
UniParc	UPI00000C54A9
UniProtKB Acc	Q9I2T4
UniProtKB ID	Q9I2T4_PSEAE
UniRef100	UniRef100_W0YP56
UniRef50	UniRef50_A0A0D0RCL5
UniRef90	UniRef90_A6V710

Product

Feature Type	CDS
Coding Frame	1
Product Name	NppA2 Product Name Confidence: Class 1
Synonyms	probable binding protein component of ABC transporter
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-2.87
Kyte-Doolittle Hydrophobicity Value	-0.489
Molecular Weight (kDa)	70.5
Isoelectric Point (pI)	6.45

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 432 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003391 (817 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA1810 Search term: nppA2 Search term: NppA2

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

The Pseudomonas aeruginosa PA14 ABC Transporter NppA1A2BCD Is Required for Uptake of Peptidyl Nucleoside Antibiotics. Pletzer D, Braun Y, Dubiley S, Lafon C, Köhler T, Page MGP, Mourez M, Severinov K, Weingart H J Bacteriol 2015 Jul;197(13):2217-2228 PubMed ID: 25917903

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799