Pseudomonas aeruginosa PAO1, PA2396 (pvdF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2396
Name
pvdF
Replicon chromosome
Genomic location 2652230 - 2653057 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251086.1
GI 15597592
Affymetrix PA2396_at
Entrez 878624
GenBank AAG05784.1
INSDC AAG05784.1
NCBI Locus Tag PA2396
protein_id(GenBank) gb|AAG05784.1|AE004665_11|gnl|PseudoCAP|PA2396
TIGR NTL03PA02396
UniParc UPI00000C56AE
UniProtKB Acc Q9I184
UniProtKB ID Q9I184_PSEAE
UniRef100 UniRef100_Q9I184
UniRef50 UniRef50_Q9I184
UniRef90 UniRef90_Q9I184

Product

Feature Type CDS
Coding Frame 1
Product Name
pyoverdine synthetase F
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.2, molecular weight:31.0, PMID:21674802)
Charge (pH 7) -8.56
Kyte-Doolittle Hydrophobicity Value -0.341
Molecular Weight (kDa) 31.0
Isoelectric Point (pI) 4.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6CUL TRANSFERASE 03/26/18 PvdF of pyoverdin biosynthesis is a structurally unique N10-formyltetrahydrofolate-dependent formyltransferase Pseudomonas aeruginosa PAO1 2.3 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB
Category Pyoverdine
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 15 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003131 (335 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2396
Search term: pvdF

Human Homologs

References

Involvement of a transformylase enzyme in siderophore synthesis in Pseudomonas aeruginosa.
McMorran BJ, Kumara HMCS, Sullivan K, Lamont IL
Microbiology (Reading) 2001 Jun;147(Pt 6):1517-1524
PubMed ID: 11390682
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802