Pseudomonas aeruginosa PAO1, PA2528 (muxA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2528
Name
muxA
Synonym: yegM
Replicon chromosome
Genomic location 2854011 - 2855291 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251218.1
GI 15597724
Affymetrix PA2528_at
Entrez 882937
GenBank AAG05916.1
INSDC AAG05916.1
NCBI Locus Tag PA2528
protein_id(GenBank) gb|AAG05916.1|AE004680_4|gnl|PseudoCAP|PA2528
TIGR NTL03PA02528
UniParc UPI00000C571F
UniProtKB Acc Q9I0V5
UniProtKB ID Q9I0V5_PSEAE
UniRef100 UniRef100_A0A069QB48
UniRef50 UniRef50_Q48IF0
UniRef90 UniRef90_W0YTB6

Product

Feature Type CDS
Coding Frame 1
Product Name
MuxA
Product Name Confidence: Class 1
Synonyms probable RND efflux membrane fusion protein precursor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.25
Kyte-Doolittle Hydrophobicity Value -0.184
Molecular Weight (kDa) 45.3
Isoelectric Point (pI) 9.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MuxA MuxA is a membrane fusion protein component of the efflux pump system MuxABC-OpmB in Pseudomonas aeruginosa. [PMID:19713238] ARO:3004073 protein homolog model Perfect 803.1 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Drosophila melanogaster
Notes
Infection model Drosophila melanogaster infection
Reference 21369987
Source 2
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Brassica pekinensis
Notes
Infection model Brassica pekinensis infection
Reference 21369987

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 433 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003039 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2528
Search term: muxA
Search term: MuxA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa.
Mima T, Kohira N, Li Y, Sekiya H, Ogawa W, Kuroda T, Tsuchiya T
Microbiology (Reading) 2009 Nov;155(Pt 11):3509-3517
PubMed ID: 19713238