Pseudomonas aeruginosa PAO1, PA2656 (bqsS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2656
Name
bqsS
Synonym: carS
Replicon chromosome
Genomic location 3006005 - 3007342 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentFirst described as BqsS in PMID:19513205 (2008). Later referred to as CarS in PMID:26755627 (2016). According to pseudomonas.com guidelines, earlier gene name takes precedence if it appeared in a peer-reviewed scientific publication.

Cross-References

RefSeq NP_251346.1
GI 15597852
Affymetrix PA2656_at
Entrez 882365
GenBank AAG06044.1
INSDC AAG06044.1
NCBI Locus Tag PA2656
protein_id(GenBank) gb|AAG06044.1|AE004694_7|gnl|PseudoCAP|PA2656
TIGR NTL03PA02656
UniParc UPI00000C5790
UniProtKB Acc Q9I0I2
UniProtKB ID Q9I0I2_PSEAE
UniRef100 UniRef100_W0YRW2
UniRef50 UniRef50_C1DL27
UniRef90 UniRef90_A0A086BZ01

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component sensor BqsS
Product Name Confidence: Class 1
Synonyms probable two-component sensor
two-component sensor CarS
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.93
Kyte-Doolittle Hydrophobicity Value -0.251
Molecular Weight (kDa) 49.8
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 602 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002901 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2656
Search term: bqsS

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
The Pseudomonas aeruginosa PAO1 Two-Component Regulator CarSR Regulates Calcium Homeostasis and Calcium-Induced Virulence Factor Production through Its Regulatory Targets CarO and CarP.
Guragain M, King MM, Williamson KS, Pérez-Osorio AC, Akiyama T, Khanam S, Patrauchan MA, Franklin MJ
J Bacteriol 2016 Jan 11;198(6):951-63
PubMed ID: 26755627
A novel two-component system BqsS-BqsR modulates quorum sensing-dependent biofilm decay in Pseudomonas aeruginosa.
Dong YH, Zhang XF, An SW, Xu JL, Zhang LH
Commun Integr Biol 2008;1(1):88-96
PubMed ID: 19513205
The ferrous iron-responsive BqsRS two-component system activates genes that promote cationic stress tolerance.
Kreamer NN, Costa F, Newman DK
mBio 2015 Feb 24;6(2):e02549
PubMed ID: 25714721
BqsR/BqsS constitute a two-component system that senses extracellular Fe(II) in Pseudomonas aeruginosa.
Kreamer NN, Wilks JC, Marlow JJ, Coleman ML, Newman DK
J Bacteriol 2012 Mar;194(5):1195-204
PubMed ID: 22194456