Pseudomonas aeruginosa PAO1, PA2862 (lipA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2862
Name
lipA
Replicon chromosome
Genomic location 3214282 - 3215217 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251552.1
GI 15598058
Affymetrix PA2862_lipA_at
DNASU PaCD00007248
Entrez 882662
GenBank AAG06250.1
INSDC AAG06250.1
NCBI Locus Tag PA2862
protein_id(GenBank) gb|AAG06250.1|AE004712_10|gnl|PseudoCAP|PA2862
TIGR NTL03PA02862
UniParc UPI0000031212
UniProtKB Acc P26876
UniProtKB ID LIP_PSEAE
UniRef100 UniRef100_P26876
UniRef50 UniRef50_P26876
UniRef90 UniRef90_P26876

Product

Feature Type CDS
Coding Frame 1
Product Name
lactonizing lipase precursor
Product Name Confidence: Class 1
Synonyms triacylglycerol lipase
Evidence for Translation
Charge (pH 7) -0.39
Kyte-Doolittle Hydrophobicity Value -0.008
Molecular Weight (kDa) 32.7
Isoelectric Point (pI) 6.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1EX9 HYDROLASE 05/02/00 CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE Pseudomonas aeruginosa 2.54 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002707 (468 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2862
Search term: lipA

Human Homologs

References

Purification and properties of extracellular lipase from Pseudomonas aeruginosa EF2.
Gilbert EJ, Cornish A, Jones CW
J Gen Microbiol 1991 Sep;137(9):2223-9
PubMed ID: 1748875
Purification, molecular cloning, and expression of lipase from Pseudomonas aeruginosa.
Chihara-Siomi M, Yoshikawa K, Oshima-Hirayama N, Yamamoto K, Sogabe Y, Nakatani T, Nishioka T, Oda J
Arch Biochem Biophys 1992 Aug 1;296(2):505-13
PubMed ID: 1632642